Introduction of a real-time PCR instrument in the Debrecen laboratory of the National Food Chain Safety Office for Salmonella detection
Author: Xénia Erika Ozsváth, Ibolya Keszler, Karolina Rigó, Károly Pál
Salmonellosis is one of the most widespread zoonoses, being the animal disease spreading to humans encountered most often all over Europe . Consequently, the detection of Salmonella spp. in foods can be classified as a microbiological analysis of primary importance in the Debrecen Regional Food Chain Laboratory of the National Food Chain Safety Office, using the horizontal method. However, the Salmonella detection method according to standard MSZ EN ISO 6579-1:2017 requires a long time, at least 3 working days. Therefore, it was decided in the Debrecen Regional Food Chain Laboratory of the National Food Chain Safety Office to introduce a rapid method based on the use of a real-time PCR instrument. The applicability of the method was tested on the basis of the results of Salmonella detection in meat samples. There was little difference when comparing the results obtained using horizontal and molecular biological methods. The results of the two methods were 99% identical. Considering the efficiency of real-time PCR (99.3%), encouraging conclusions can be drawn regarding the introduction of the new method.
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